Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 14.5, Problem 1MI
MICRO INQUIRY
Relative to each promoter, where would you predict phosphorylated OmpR would bind the ompC and ompF genes?
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Give typing answer with explanation and conclusion
a) List three eukaryotic gene expression mechanisms that do not occur in prokaryotes. For two of these, give specific examples and the functional outcomes.
b) Describe what is meant by the term “RNA silencing”.
c) Using diagrams, give two examples of RNA silencing mechanisms and indicate one difference.
Explain how HOTAIR plays a role in the transcriptional regulationof particular genes.
Expression of a cloned eukaryotic gene in a bacterial cellinvolves many challenges. The use of mRNA and reversetranscriptase is part of a strategy to solve the problem of(A) post-transcriptional processing.(B) post-translational processing.(C) nucleic acid hybridization.(D) restriction fragment ligation
Chapter 14 Solutions
Prescott's Microbiology
Ch. 14.2 - MICRO INQUIRY In what way is on inducer molecule...Ch. 14.2 - MICRO INQUIRY Is allolactose a corepressor or...Ch. 14.2 - Retrieve, Infer, Apply Many genes and operons are...Ch. 14.2 - Prob. 2CCCh. 14.2 - Prob. 3CCCh. 14.3 - MICRO INQUIRY How does this attenuation respond to...Ch. 14.4 - MICRO INQUIRY How does inhibition of translation...Ch. 14.4 - Prob. 1CCCh. 14.4 - Prob. 2CCCh. 14.4 - Prob. 3CC
Ch. 14.4 - Retrieve, Infer, Apply How are translational...Ch. 14.4 - Prob. 5CCCh. 14.5 - MICRO INQUIRY Relative to each promoter, where...Ch. 14.5 - MICRO INQUIRY For what other compounds would you...Ch. 14.5 - Prob. 1CCCh. 14.5 - Retrieve, Infer, Apply What is diauxic growth?...Ch. 14.5 - Retrieve, Infer, Apply Describe the events that...Ch. 14.5 - Retrieve, Infer, Apply E. coli has two phosphate...Ch. 14.5 - Prob. 5CCCh. 14.6 - Prob. 1MICh. 14.6 - MICRO INQUIRY Why does V. harveyi make three...Ch. 14.6 - Prob. 3MICh. 14.6 - Prob. 1CCCh. 14.6 - Prob. 2CCCh. 14.6 - Prob. 3CCCh. 14.6 - Retrieve, Infer, Apply Why might bacteria use...Ch. 14.6 - Prob. 5CCCh. 14.6 - Prob. 6CCCh. 14 - Prob. 1RCCh. 14 - Prob. 2RCCh. 14 - Prob. 3RCCh. 14 - Prob. 4RCCh. 14 - Attenuation affects anabolic pathways, whereas...Ch. 14 - Describe the phenotype of the following E. coli...Ch. 14 - Prob. 3ALCh. 14 - What would be the phenotype of a B. subtilis...Ch. 14 - Propose a mechanism by which a cell might sense...Ch. 14 - Prob. 6ALCh. 14 - Prob. 7ALCh. 14 - Prob. 8ALCh. 14 - Prob. 9AL
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a) The best vector to use determine receptor binding protein expression would have been one with a GFP gene (green flourescent protein) attached. State one (1) reason why including this gene would have made the experiment easier and whether you have inserted the receptor binding domain gene before or after the GFP gene. b) You wish to determine the sucess pf your transformation by detecting the presence of receptor binding domian mRNA. Describe the key steps of the hybrdization technique you would use, clearly stating how you would design the probe to detect your receptor mRNA.arrow_forwardConsider the gal10D56 reporter gene. In 300 words or fewer, describe 1) the role of GAL7 in galactose metabolism and its importance for cell function 2) the mutation present in the gal10D56 reporter gene 3) the consequence of this mutation for GAL7 expression in wild type cells, 4) the mechanism by which certain mutations can suppress the effects of gal10D56, and 5) the specific purpose for using this reporter gene.arrow_forwardEnhancer RPA Gene A Use the diagram above, which depicts a chromosomal region less than 500,000 bp, to determine the expression of each gene under the scenarios specified in the table. Regulatory promoters are indicated by "RP". Genes A, B, and C have intermediate expression when no regulatory proteins are bound. Complete the table by choosing high, intermediate, or low to describe the expression level of each gene under each specified scenario (i and ii). No regulatory proteins bound (i) A repressor binds to RPA and an activator binds to RPC (ii) A regulatory protein binds at each of the activator, insulator, and silencer. Expression of A? Intermediate 1. [Select] 4. [Select] Insulator RPB Expression of B? 2. Gene B Intermediate 5. [Select] [Select] Silencer RPC Expression of C? 3. Gene C Intermediate 6. [Select] [Select]arrow_forward
- explain please: Spike-Driven Syncytia Formation Is Coupled to S20 Fragment Generation in the Presence of ACE2. To investigate the functional and biochemical signatures of spike (S) protein upon engaging its receptor ACE2 in live cells, we first utilized a cell–cell fusion assay to obtain potentially cleaved spike protein products from syncytia . In this system, HEK293T cells transfected with plasmids encoding WT spike readily formed syncytia after coculturing with HEK293T cells expressing human and Vero E6-ACE2, as well as colorectal adenocarcinoma Caco-2 and lung adenocarcinoma Calu-3 cells expressing endogenous ACE2. We then collected these adherent syncytia and immunoblotted for cleaved spike species using a rabbit polyclonal antibody specifically detecting the S2 ectodomain through standard reducing Tris-glycine sodium dodecyl sulfate polyacrylamide gel electrophoresis. This experiment revealed that spike expression in HEK293T cells displayed full-length S and autocleaved S2…arrow_forwardPlease answer fast If the plasmid Lac operon has a mutated lacO operator gene that prevents the repressor from binding, which of the following will occur when lactose is absent? No beta-lactamase will be produced. A functional beta-lactamase will be produced. A non-functional beta-lactamase will be produced. Both a functional and a non-functional beta-lactamase will be produced. If the plasmid Lac operon has a mutated promotor that prevents RNA polymerase from binding, which of the following will occur when lactose is present? No beta-lactamase will be produced. A functional beta-lactamase will be produced. A non-functional beta-lactamase will be produced. Both a functional and a non-functional beta-lactamase will be produced.arrow_forwardMeasure the uptake of leucine by epithetial cells of the mouse intestine. Measurements of the rate of update of L-leucine, D-Leucine, and L-valine , with and without Na+ in the assay were perform and yield different results (see table below). A) What can you conclude about the properties and mechanism of leucine transporter? B) Would you expect L-leucine uptake to be inhibited by Ouabain, which is a cardiac glycoside drug treatment?arrow_forward
- Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator +1 lac Y lac A DNA 1. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 2. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 3. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 4. Why is it adaptive for a bacterium to not express the genes that encode for that lactose utilization proteins when lactose is not available or when glucose is present? 5. Why is it adaptive for the structural genes for using lactose to be under the control of a single promoter, i.e., synthesize a polycistronic message rather than three monocistronic message?arrow_forwardRead aloud V Draw Highlight 2. You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase). (a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities. (b) You identify another loss-of-function mutation with the following expression pattern: Media + glucose - lactose + glucose +lactose - glucose - lactose - glucose + lactose lacZ expression Low Low High High What components of the lac operon could be mutated to produce this phenotype? List all possibilities.arrow_forward8:20 ■■ LTE < Spring 2024 - Senior Comprehensives (... Question 6ɔ (ividitudtory) In the Lac operon, what happens when allolactose attaches to the repressor protein? a) b) c) the repressor is induced to attach to the promoter RNA polymerase is induced to attach to the operator lactose synthesis enzymes are produced by genes Z, Y,A d) the repressor is inactivated Question 88 (Mandatory) What is the purpose of X-chromosome inactivation in mammals? a) It accomplishes dosage compensation. b) It assists in the interactions beteeen genes Tra and Dsx. c) It prevents trisomy from occurring. d) It produces the testis determination factor. Question 94 (Mandatory) Duchenne muscular dystrophy is inherited in an X- linked recessive pattern. A female is known to be heterozygous for Du muscular dystrophy XD xdarrow_forward
- If all members of a transposable element family can besilenced by dsRNA synthesized from a single familymember, how is it possible for one element family (likeTc1) to have 32 copies in the C. elegans genome whileanother family (Tc2) has fewer than 5 copies?arrow_forwardList and briefly explain. C-terminal domain of RNA polymerase II function to ensure that the varoius sets of mRNA processing enzymes carry out their duties at the apporpiate time and place?arrow_forwardIs RNA polymerase saturated with substrates in vivo? Describe experiments that might indicate whether RNA polymerase is operating at Vmax with respect to its nucleotide substrates.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Industrial Processes and By-products | 9-1 GCSE Chemistry | OCR, AQA, Edexcel; Author: SnapRevise;https://www.youtube.com/watch?v=CMLKgqEMXwc;License: Standard Youtube License