write a detailed description of each of the following tests/media: a. TSI Agar Slant b. SIM Medium c. Urease Test d. The IMViC Tests e. The Catalase Test f. The Oxidase Test
Q: For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E…
A: EnteroPluri-Test is a 12-sector system that consists of special culture media. which allows…
Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…
A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…
Q: Match the test with the appropriate reagent/ chemical a. DNA test b. Paper chromatography c.…
A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…
Q: CITRATE UTILIZATION TEST WAS PERFORMED IN A CULTURE MEDIA WHICH CONTAIN A STRONG BUFFER. AND EVEN A…
A: In this test citrate agar is used, where citrate is carbon source and NH4and ability of organism to…
Q: What are the advantages of the API 20E test compared to regular biochemical tube media
A: API 20E test helps in the determination of bacteria from lab-scale activated sludge treatment…
Q: The following media are used to grow bacteria for various purposes. Match the examples of media that…
A: MacConkey Agar: - Selective Growth Medium Malt Extract Agar:- Complex Medium
Q: Explain why the following steps are essential during subculturing:a. Flaming the inoculating…
A: A microbial culture made by transferring the cells from the previous/old culture medium into a fresh…
Q: In product testing, microbial growth is found.| Interpretation: Rationale:
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Match the test with a positive result a. DNA test b. Paper chromatography test c. Celllar…
A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…
Q: Choose the combination of answers that most accurately completes the statement.Microbe(s) that…
A: The unicellular minute organisms which are not visible from the naked eye are known as microbes.…
Q: In a Kligler Iron Agar/Triple Sugar Iron test you end up with a result with a yellow butt and red…
A: When a non-lactose fermenting bacterium hydrolyses the agar slant in the Kliger Iron Agar/ Triple…
Q: 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating…
A: Agar is formed by two components, agarose, and agaropectin molecules. Agar is used as culture media…
Q: Which of the following is true of the phenol coefficient test? a. It is particularly reliable for…
A: Antibiotics are the medicines which are used to cure infections which are caused by bacteria. These…
Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…
A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…
Q: Which of the following is TRUE about the Nitrate Reduction test? Question 1 options: A Nitrate…
A: According to the question, we have to mention Which of the following is TRUE about the Nitrate…
Q: Milk is usually pasteurized by a. the high-temperature short-time method c. batch method b.…
A: Pasteurization is the process, in which bacteria are killed and the sterilization is done to improve…
Q: DNA fingerprinting and antibody-based ELISA tests would be used during which step of microbial…
A: There are many techniques that are very helpful in the field of biotechnology such as polymerase…
Q: Which of the following specimens must be removed by steriletechniques?a. feces b. urine c. upper…
A: Sterile techniques are very necessary in extraction of sample for testing. So, there are several…
Q: discuss how the black precipitate is formed in the butt of the test tube during a tripple sugar iron…
A: Triple sugar iron fermentation test is a microbiological test, that test a microorganism’s ability…
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A: Microbial control indicates the prevention or inhibition of microbial growth. It involves physical…
Q: The catalase test is used to primarily differentiate Staphylococi from _____________. A.…
A: The enzyme, catalase, is produced in the bacteria that respire using oxygen. The main role of the…
Q: An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is…
A: An inoculation loop is a tool used by microbiologists to transfer a small culture of microorganisms.…
Q: Which of the results shown in the figure (Test A, B, or C) used Biuret Reagent? TEST A TEST B TEST C…
A: Peptides are defined as a short string of amino acids where it contains only 2 to 50 amino acid…
Q: Which is statement below is the correct definition of the Sterility Assurance Limit (SAL). Select…
A: Sterilization and pasteurization are the two methods of destroying harmful microbes. The…
Q: Which statement is supported by the experiment below? Select all the apply. 1% NaCl agar plate 6.5%…
A: Here, both S.aureus and E.coli can grow in normal salt concentration S.aureus can tolerate…
Q: Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.
A: Aspetic techniques that you should follow during inoculating a broth from a plate culture are:…
Q: You inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and…
A: MacConkey agar is a selective differential indicator to isolate gram-negative and enteric bacteria.…
Q: 1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C.…
A: The removal of any microorganism or viable spores or bacteria or viruses from the surface. The…
Q: Which of the following medium would be most appropriate for the bacterial cultures from stomach? a.…
A: Microorganism culture out of their natural environment in pure form in the laboratory has been…
Q: From figure shows triplesugar iron agar. What biochemical characteristics does this…
A: “Triple sugar iron test” is one of the “biochemical tests” used to test the ability of microbe in…
Q: Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big…
A: Petri plates, slants and stabs of Culture media such as Luria Bertani agar is used in microbiology…
Q: If you streak a sample from your nose onto a Mannitol salt agar plate and the medium turns yellow,…
A:
Q: Which of the following factors affect the effectiveness of hand washing: Select one: a. Frequency…
A: Pathogens are microorganisms that can cause an infection and disease to an organism. Our hands are…
Q: What type of medium is best used to maintain a preserve specimen before clinical analysis? A.…
A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…
Q: Explain with the examples of biochemical test that are used to identify the bacteria in the…
A: The smallest organism that is usually defined that the are been can be seen with the naked eye is…
Q: what information about the identity and physiology of a bacterium or yeast can be learned from…
A: Micro organisms are grown in a solid medium (agar) with supplements as a primary step in order to…
Q: Which of the following is not one of the six “I’s”? a. inspection d. incubation b. identification e.…
A: Microbes: Microbes are not visible to the naked eye and they grow in complex associations.…
Q: Explain the concept of and apply the aseptic technique
A: Bacteria, viruses, and fungi are the microorganism that is present everywhere. Some of them cause…
Q: Give the use and advantages of the following cultures for the examination of colony characteristics:…
A: Culture media: a. Culture media provides an environment that supplies all essential nutrition for…
Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…
A: A mixed culture is one that contains more than one type of organism growing in a sterile medium. The…
Q: What is the test that is used to demonstrate pasteurisation
A: Ans-phosphatase test is used to demonstrate pasteurization. Dairies and food processors can use…
Q: You are testing for Escherichia coli in the sample. What media would you use? and Describe typical…
A: A medium in microbiology is defined as a solid or liquid substance used to culture the…
Q: Explain why desiccation and cold are not reliable methods of disinfectionbut how they can still be…
A: Sterilization is a technique that removes, kills, or deactivates all forms of life and other…
Q: Clear tubes in the MIC test were inoculated in Mueller-Hinton Agar to determine the MBC of the…
A: MBC - stands for minimum bactericidal concentration It is the lowest concentration of antibiotics…
Q: Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different…
A: The liquid or solid substratum on which, the organ or cell explants can be grown. The medium…
Q: When the new batch of culture media is prepared, why it is recommended to test the AST using the…
A: Introduction Culture is referred to the process of growing the cell outside the animal or plant…
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- The Guthrie Test: Uses an agar that is deficient in one growth factor for Bacillus subtilis, which inhibits the growth of Bacillus, and if the factor is present in high а. enough concentration in the patient sample, this allows the Bacillus to grow b. Is used to determine the growth characteristics of all Bacillus species O . Is not used in any test that I know of Uses an agar that contains all growth factors for Bacillus subtilis, and if patient sample has high enough concentration of one inhibitory factor in their blood, the Bacillus does not growWhich type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infectionFor Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box provided Test Media Needed
- For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E System for Enterobacteriaceae identification. a. time requirement b. specimen preparation c. tests utilized d. anaerobic conditions e. interpretation of resultsGive the use and advantages of the following cultures for the examination ofcolony characteristics: a. Agar plateb. Agar slantc. Broth culturesA 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?
- In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar plates b. Micropipettor c. Inoculating needle d. Alcohol lamp or Bunsen burner e. Test tubes or microcentrifuge tubesWhich of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive testExplain the mechanism leading to the given reactions in the inoculated biochemical media. 1. Blackening of the medium in Triple Sugar Iron Test slant2. Development of pink to red color in nitrate broth in Nitrate Reduction Test
- Choose one specific way from each of the two methods of inhibiting the growth of microbes below. Describe the method, explain the mechanism of action of killing the microorganisms and give practical applications of your chosen ways. 1. Physical Methods - Heat, cold, dessication, radiation, ultrasonic waves, filtration, gaseous atmosphere 2. Chemical Methods- disinfectants, antisepticsMEDIA. Match the name of the medium with the physiological test. A medium may be used more than once. Tests may require more than one answer. a. Kligler’s iron agar b. MR-VP broth c. phenol red lactose d. SIM medium e. Simmon’s citrate agar f. skim milk agar g. spirit blue agar h. tryptone broth 2,3-butanediol fermentation carbohydrate fermentation casein hydrolysis citrate utilization hydrogen sulfide production mixed-acid fermentation triglyceride hydrolysis tryptophan degradationIf the concentration of solutes in the fluid surrounding the cell is greater than the cell's cytoplasm then the surrounding fluid is to the cell cytoplasm. 00000 Endergonic Exergonic Hypertonic Isotonic Hypotonic