Someone submits a saliva sample for a PCR test to determine whether he is infected withSARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA isconverted into cDNA.A. Why is this conversion important B. What is the first enzyme required for this step? C. Does this enzyme require a primer? Briefly explain. D. Three days later, the person submits another sample. The qPCR data againindicates a positive result; however, the spike gene Ct value is now 33. Based on the PCRdata, are they at the beginning or end of a COVID viral infection? Explain how you made thisdetermination
Someone submits a saliva sample for a PCR test to determine whether he is infected withSARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA isconverted into cDNA.A. Why is this conversion important B. What is the first enzyme required for this step? C. Does this enzyme require a primer? Briefly explain. D. Three days later, the person submits another sample. The qPCR data againindicates a positive result; however, the spike gene Ct value is now 33. Based on the PCRdata, are they at the beginning or end of a COVID viral infection? Explain how you made thisdetermination
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Someone submits a saliva sample for a PCR test to determine whether he is infected with
SARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA is
converted into cDNA.
A. Why is this conversion important
B. What is the first enzyme required for this step?
C. Does this enzyme require a primer? Briefly explain.
D. Three days later, the person submits another sample. The qPCR data again
indicates a positive result; however, the spike gene Ct value is now 33. Based on the PCR
data, are they at the beginning or end of a COVID viral infection? Explain how you made this
determination
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