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- are there any points within the linear range of a signal response curve that cannot be used to determine the concentration of analyte in a sample?detector Answer Bank emission monochromator excitation monochromator light source sample cell ^ex A em It is typically a grating, prism, or filter that selects a narrow wavelength band of radiation and passes it to the sample. It holds the sample and has a defined pathlength. It is typically a photomultiplier tube that generates an electric signal when struck by photons. It is positioned 90° to the incident light and selects a narrow wavelength band of radiation to pass toAn analyst needs to evaluate the potential effect of an interferent, I, on the quantitative analysis for an analyte, A. She begins by measuring the signal for a sample in which the interferent is absent and the analyte is present with a concentration of 15 ppm, obtaining an average signal of 23.3 (arbitrary units). When analyzing a sample in which the analyte is absent and the interferent is present with a concentration of 25 ppm, she obtains an average signal of 13.7. What is the sensitivity for the analyte? What is the sensitivity for the interfering? What is the value of the selectivity coefficient? What is the maximum concentration of interferent relative to that of the analyte (i.e. interferent/analyte), if the error in the analysis is to be less than 1%?
- An analyst needs to evaluate the potential effect of an interferent, I, on the quantitative analysis for an analyte, A. She begins by measuring the signal for a sample in which the interferent is absent and the analyte is present with a concentration of 15 ppm, obtaining an average signal of 23.3 (arbitrary units). When analyzing a sample in which the analyte is absent and the interferent is present with a concentration of 25 ppm, she obtains an average signal of 13.7.Match each of the definitions with the correct term. A signal B standardization C analyte D stock solution E assay F noise G qualitative analysis H baseline 1 Limit of Detection. J quantitative analysis K matrix L secondary standard M uncertainty N Limit of Quantitation O attenuation P calibration standards Q aliquot R blank. S primary standard making a signal smaller in amplitude a sample that contains the same matrix as the analyte sample, but contains no analyte the process by which the concentration of the analyte in a secondary standard is determine a method of analysis that determines the amount of an analyte the instrument response associated with the presence of an analyte a substance that may not be highly purified, but can be standardized with a primary standard and use to calibrate an instrument Submit Answer Tries 0/12 Post DiscussionA 10.00-g sample containing an analyte was transferred to a 250-mL volumetric flask and diluted to volume. When a 10.00-mL aliquot of the resulting solution was diluted to 25.00 mL it was found to give a signal of o.235 (arbitrary units). A second 10.00-mL aliquot was spiked with 10.00 mL of a 1.00- ppm standard solution of the analyte and diluted to 25.0o mL. The signal for the spiked sample was found to be o.502. Calculate the weight percent of analyte in the original sample.
- A 10.00 g sample containing an analyte was transferred to a 250 mL volumetric flask and diluted to volume. When a 10.00 mL aliquot of the resulting solution was diluted to 25.00 mL it was found to give a signal of 0.235 (arbitrary units). A second 10.00 mL aliquot was spiked with 10.00 mL of a 1.00 ppm standard solution of the analyte and diluted to 25.00 mL. The signal for the spiked sample was found to be 0.502. Calculate the weight percent of analyte in the original sample.What are the main components of a spectrophotometer? Choose all that applies. radiation source sample compartment/holder monochromator detectorYou are measuring the concentration of an unknown protein sample, and the absorbance at 595 nm (or A595) of your unknown is greater than that of the highest-concentration standard solution. It is not advisable to simply extrapolate the line of the standard curve to calculate the concentration of the unknown In this scenario, what would be the best strategy to measure the concentration of your unknown (instead of extrapolating from the standard curve)? (answer in 5 sentences)
- 3) The solution taken from a factory waste sample to prepare an internal standard contains Pb + 2 and Ag + 2, and the analysis peaks were determined as 298 mAU and 324 mAU. To analyze the unknown, a solution containing 0.150 M, 50.0 mL of Ag + 2 is put into the unknown liquid and completed to 100.0 mL. When the peaks are measured again, 670 mAU and 856 mAU signals are read from the samples. What is the concentration of Pb + 2 in the unknown liquid?The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.A 600 MHz NMR has a signal to noise ratio of 1000 for a proton standard. The 400 MHz NMR has a signal to noise ratio of 200 for a proton standard. If it takes 20 minutes to get an acceptable signal to noise ratio for a sample on the 600 MHz NMR, how long will it take you to achieve the same signal to noise ratio on the 400 MHz NMR?