Calculate the molecular weight of protein in sample A. Given the protein migration distance is 26 mm and the migration distance of the dye front (gel length) is 6.5 cm.
Calculate the molecular weight of protein in sample A. Given the protein migration distance is 26 mm and the migration distance of the dye front (gel length) is 6.5 cm.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
1. Calculate the molecular weight of protein in sample A. Given the protein migration distance is 26 mm and the migration distance of the dye front (gel length) is 6.5 cm.
![2.5
y = -1.7004x +2.3693
R = 0.9918
.5
0.1
0.2
0.3
0.4
0.5
Rf
Figure 1/ Rajah 1
[b] Figure 1 presents the standard curve of a protein molecular weight (MW) marker in a SDS-PAGE experiment. A researcher used this graph
to determine the molecular weight of two types of protein in sample A and B.
log MW (kDa)
2.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4b3e384e-5f91-4be8-aca6-520832fab52e%2F66eb39cd-d9dd-4c86-a265-c9a0ae83ac3b%2Fasb0rha_processed.jpeg&w=3840&q=75)
Transcribed Image Text:2.5
y = -1.7004x +2.3693
R = 0.9918
.5
0.1
0.2
0.3
0.4
0.5
Rf
Figure 1/ Rajah 1
[b] Figure 1 presents the standard curve of a protein molecular weight (MW) marker in a SDS-PAGE experiment. A researcher used this graph
to determine the molecular weight of two types of protein in sample A and B.
log MW (kDa)
2.
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