Black men are more likely to get prostate cancer than other men. You are working on a prostate cancer project. You want to isolate cancer cells from a diseased prostate and culture the cells. Akilah, your lab partner uses a procedure to isolate the prostate cancer cells. You decide you want to culture the cells for various types of biological analysis. Write up the procedure used to culture the prostate cancer cells You froze some of the prostate cancer cells for future experiments. How did you freeze the cells?Now you and your team decided to analyze the genetic material. Your lab mate isolated the DNA from the prostate cancer cells. You isolated the RNA from the prostate cancer cells. Write up the procedure you used to isolate the RNA Your lab mate does Northern blotting to visualize the RNA expression. You are interested in two genes that are overexpressed in prostate cancer cells. Write up or illustrate the procedure you used to run the Northern blot in your own words.Alex, another student informed you about another procedure called single-cell RNA sequencing. You are intrigued! Why? Your collaborator from Fisk University has isolated proteins from healthy prostate cancer cells. You want to compare and contrast the two protein expressions from part 4. Write up the procedure to examine protein expression from the prostate cancer cells and healthy prostate cancer cells.You are interested in seeing where the two proteins are located in the cells. You order antibodies that recognize the proteins. You ask a high school student working with you to do the procedure. Write up the procedure to investigate protein localization in cells.Ok, now from parts 5 and 6 you have the protein expression data from the prostate cancer cells and healthy prostate cells. Next, you want to knock out or silence the gene for the proteins to see if this prevents prostate cancer. Write up the procedure to knock out or silence the genes. Please indicate if the procedure is knocking out or silencing the genes.
Black men are more likely to get prostate cancer than other men. You are working on a prostate cancer project. You want to isolate cancer cells from a diseased prostate and culture the cells. Akilah, your lab partner uses a procedure to isolate the prostate cancer cells. You decide you want to culture the cells for various types of biological analysis. Write up the procedure used to culture the prostate cancer cells You froze some of the prostate cancer cells for future experiments. How did you freeze the cells?Now you and your team decided to analyze the genetic material. Your lab mate isolated the DNA from the prostate cancer cells. You isolated the RNA from the prostate cancer cells. Write up the procedure you used to isolate the RNA
Your lab mate does Northern blotting to visualize the RNA expression. You are interested in two genes that are overexpressed in prostate cancer cells. Write up or illustrate the procedure you used to run the Northern blot in your own words.Alex, another student informed you about another procedure called single-cell RNA sequencing. You are intrigued! Why?
Your collaborator from Fisk University has isolated proteins from healthy prostate cancer cells. You want to compare and contrast the two protein expressions from part 4. Write up the procedure to examine protein expression from the prostate cancer cells and healthy prostate cancer cells.You are interested in seeing where the two proteins are located in the cells. You order antibodies that recognize the proteins. You ask a high school student working with you to do the procedure. Write up the procedure to investigate protein localization in cells.Ok, now from parts 5 and 6 you have the protein expression data from the prostate cancer cells and healthy prostate cells. Next, you want to knock out or silence the gene for the proteins to see if this prevents prostate cancer. Write up the procedure to knock out or silence the genes. Please indicate if the procedure is knocking out or silencing the genes.
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