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- 8. Hypercholesterolemia is a frequent complication of diabetes mellitus in patients with prolonged hyperglycemia. Why a high level of glucose in blood causes hypercholesterolemia and atherosclerosis? For the answer explain: a) how glucose can interact with the proteins and the consequences of this reaction for the proteins; b) glycation of which proteins results in hypercholesterolemia; c) possible causes and complications of hypercholesterolemia.6. A. List all of the ionizable functional groups that are found in insulin when in aqueous solution. List which amino acid residues have these ionizable groups and list all of the pka and pKb values (including the R groups) that are on both polypeptide chains that make up insulin. (see the table at the end of this HW set; note that tyrosine and cysteine both have unusual pka's, since these side groups ionize above the pKR's given to have a negative charge). B. The isoelectric point of insulin is reported to be around 5.3-5.35. Using the method covered in class, estimate the isoelectric point of insulin and compare your answer to the values above. C. For a polypeptide to be soluble in an aqueous solution, is it good to be near the isoelectric point? Why or why not? Notes: a couple of unusual R group's that ionize (cysteine and tyrosine have R groups that have pka values; histidine has a pkb). The table at the end of this homework set (also in the lecture notes) lists the pka's and…What is the weight of epinephrine (in grams) required to make the prescription? What is the volume of water (in milliliters) needed to make the preparation isotonic?
- 8. Patients on Irinotecan therapy increase production of hCES2 even though hCES2 breaks it down. (10.1093/inci/div132) Knowing this, How would you think about determining the optimum dosage of both drugs (Irinotecan and Compound 24) in a patient with chronic diarrhea/ulcerative colitis that is being treated for colon cancer? (There are a lot of right answers here). (Remember, Irinotecan and Compound 24 are BOTH binding hCES2).1) Draw a chromatogram depicting separation of proteins 1, 2, and 3 (protein #1, mw 30,000, protein #2, mw 60,000, and protein #3, mw 90,000). b)Indicate on the chromatogram in (a) where insulin would elute. Would insulin have total accessibility, some accessibility or no accessibility to the particle pores on this column? c)Referring to the size exclusion chromatogram in (a), indicate on the chromatogram where you would expect a molecule with a size of 40,000 to elute. Would you expect the molecule to be well resolved, somewhat resolved, or not resolved from the peak for protein #1?5) Explain the synthesis, release and pharmacology of histamine
- 188. A 3-year-old boy is brought to the physician because of a 6-month history of a sway back when standing. His brother had a history of muscle weakness and died of pneumonia at the age of 12 years. Physical examination shows hip girdle weakness. His serum creatine kinase concentration is markedly increased. This patient's symptoms are most likely caused by the accumulation of a defective protein in which of the following locations in muscle cells? A) Cytoplasm B) Lysosome C) Mitochondria D) Nuclear membrane E) Peroxisome F) Smooth endoplasmic reticulum hBefore their dialysis treatment, some patients have a Creatinine level exceeding 500 umol/L (Normal Range is approximately around 62-106 umol/L). Why do you think this happens?43. Explain the synthesis steps for the major metabolic hormone in the human body.
- 11. Hydrophobic primary messengers usually cause molecular changes leading to modifications in metabolism. Examples of hydrophobic primary messengers are Explain what are their general chemical properties. A) thyroxine (tyrosine-derived hormone) and insulin (a small peptide hormone) B) the steroid hormones C) tyrosine-derived epinephrin and norepinephrin serotonin and melatonin (tryptophan-derived mammalian neurotransmitters) E) auxins (tryptophan-derived plant hormones) Answer: Explanation:A newly discovered hormone contains four amino acids linked together. Under which chemical class would this hormone be classified? lipid-derived hormone amino acid-derived hormone peptide hormone glycoproteinAlthough there are many different hormones in the human body, they can he divided into three classes based on their chemical structure. What are these classes and what is one factor that distinguishes them?
