5. Scientists recently described a unique family of chemotactile receptors (CRs) that allows for touch-taste sensation in octopus. To identify this new type of receptor, RNA-sequencing was used to study the gene expression profiles in various tissues. A. How could the investigators target mRNA (over ribosomal and other noncoding RNAs in the cell) for cDNA synthesis? B. In order to make cDNA, which three enzymes would be needed? eye skin brain Co sucker sensory epithelium statocyst olfactory organ van Giesen et al., 2020, Cell 183, 594-604 -10123 expression brain eye skin statocyst sucker olfactory org sensory ep C. Figure A visually represents the expression profile of mRNA molecules. In what tissue are transcripts encoding CRs the most enriched? D. Do you predict that in addition to mRNA, CR proteins are also enriched in this tissue? Explain your answer. NompC Touch rece CR776 CR986 CR989 CR990 CR828 CR992 CR840 CR110 CR829 CR041 CR918 CR522 CR111 CR737 chemotactile receptors (CRS)

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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5. Scientists recently described a unique family of chemotactile receptors (CRS) that allows for
touch-taste sensation in octopus. To identify this new type of receptor, RNA-sequencing was
used to study the gene expression profiles in various tissues.
A. How could the investigators target
mRNA (over ribosomal and other
noncoding RNAs in the cell) for
cDNA synthesis?
B. In order to make cDNA, which
three enzymes would be needed?
eye
skin
brain
COO
sucker
sensory epithelium
statocyst
olfactory organ
van Giesen et al., 2020, Cell 183, 594-604
A
-10123
expression
brain
eye
statocyst
skin
sucker
olfactory org
sensory ep
C. Figure A visually represents the expression profile of mRNA molecules. In what tissue
are transcripts encoding CRs the most enriched?
D. Do you predict that in addition to mRNA, CR proteins are also enriched in this tissue?
Explain your answer.
E. Propose a mechanism by which all CRs proteins in a tissue could be coordinately
regulated at the level of gene transcription.
NompC Touch receptor
CR776
CR986
CR989
CR990
CR828
CR992
CR840
CR110
CR829
CR041
CR918
CR522
CR111
CR737
chemotactile receptors (CRS)
Transcribed Image Text:5. Scientists recently described a unique family of chemotactile receptors (CRS) that allows for touch-taste sensation in octopus. To identify this new type of receptor, RNA-sequencing was used to study the gene expression profiles in various tissues. A. How could the investigators target mRNA (over ribosomal and other noncoding RNAs in the cell) for cDNA synthesis? B. In order to make cDNA, which three enzymes would be needed? eye skin brain COO sucker sensory epithelium statocyst olfactory organ van Giesen et al., 2020, Cell 183, 594-604 A -10123 expression brain eye statocyst skin sucker olfactory org sensory ep C. Figure A visually represents the expression profile of mRNA molecules. In what tissue are transcripts encoding CRs the most enriched? D. Do you predict that in addition to mRNA, CR proteins are also enriched in this tissue? Explain your answer. E. Propose a mechanism by which all CRs proteins in a tissue could be coordinately regulated at the level of gene transcription. NompC Touch receptor CR776 CR986 CR989 CR990 CR828 CR992 CR840 CR110 CR829 CR041 CR918 CR522 CR111 CR737 chemotactile receptors (CRS)
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