5. Describe three unique cell biological features of sperm cells and three unique cell biological features of egg cells. By "unique", I mean a feature that is not common in most cells. 6. You have just identified a mouse gene that is homologous to other vertebrate Fibroblast Growth Factor (FGF) genes. You do in situ hybridization and find that it is expressed in the endoderm of the early embryo. Describe the technique of in situ hybridization. Then describe three experiments you would do to study its potential function in the endoderm. Make sure to include what you would hope to discover from each experiment. 7. What are the 4 main signaling pathways that are used over and over again in embryogenesis, making sure to give the name of the ligand and receptor. Then describe why conditional knockouts would be the best way to evaluate their roles in embryogenesis.
5. Describe three unique cell biological features of sperm cells and three unique cell biological features of egg cells. By "unique", I mean a feature that is not common in most cells.
6. You have just identified a mouse gene that is homologous to other vertebrate Fibroblast Growth Factor (FGF) genes. You do in situ hybridization and find that it is expressed in the endoderm of the early embryo. Describe the technique of in situ hybridization. Then describe three experiments you would do to study its potential function in the endoderm. Make sure to include what you would hope to discover from each experiment.
7. What are the 4 main signaling pathways that are used over and over again in embryogenesis, making sure to give the name of the ligand and receptor. Then describe why conditional knockouts would be the best way to evaluate their roles in embryogenesis.
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