3. Why did a mixture of 2.00 mL of pH 7.00 phosphate buffer, 0.900 mL of deionized water and 0.100 mL of 0.0300M p-nitrophenylacetate in acetonitrile have an A400 of approximately 0.03 at zero time? (Day 1, Run 1)
3. Why did a mixture of 2.00 mL of pH 7.00 phosphate buffer, 0.900 mL of deionized water and 0.100 mL of 0.0300M p-nitrophenylacetate in acetonitrile have an A400 of approximately 0.03 at zero time? (Day 1, Run 1)
Organic Chemistry: A Guided Inquiry
2nd Edition
ISBN:9780618974122
Author:Andrei Straumanis
Publisher:Andrei Straumanis
Chapter13: Substitution
Section: Chapter Questions
Problem 8CTQ
Related questions
Question
![2. Why is there a difference between the stock solution concentration of chymotrypsin
calculated from zero time y-intercepts versus the stock solution label information?
3. Why did a mixture of 2.00 mL of pH 7.00 phosphate buffer, 0.900 mL of deionized water
and 0.100 mL of 0.0300M p-nitrophenylacetate in acetonitrile have an A400 of
approximately 0.03 at zero time? (Day 1, Run 1)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8edbc80c-85d1-4208-b7af-2db05708d335%2F8e4b2ed5-7dd0-4306-8f79-6842f285e8d6%2Fc0cilz8_processed.jpeg&w=3840&q=75)
Transcribed Image Text:2. Why is there a difference between the stock solution concentration of chymotrypsin
calculated from zero time y-intercepts versus the stock solution label information?
3. Why did a mixture of 2.00 mL of pH 7.00 phosphate buffer, 0.900 mL of deionized water
and 0.100 mL of 0.0300M p-nitrophenylacetate in acetonitrile have an A400 of
approximately 0.03 at zero time? (Day 1, Run 1)
![7. Discuss the effect of the pH change on the steady state deacylation rate; were your
observations consistent with the proposed mechanism? Explain your answer.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8edbc80c-85d1-4208-b7af-2db05708d335%2F8e4b2ed5-7dd0-4306-8f79-6842f285e8d6%2Fc065l5d_processed.jpeg&w=3840&q=75)
Transcribed Image Text:7. Discuss the effect of the pH change on the steady state deacylation rate; were your
observations consistent with the proposed mechanism? Explain your answer.
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